T4 RNA Ligase - Names and Identifiers
T4 RNA Ligase - Physico-chemical Properties
Appearance | Liquid |
Storage Condition | -20℃ |
T4 RNA Ligase - Introduction
T4 RNA Ligase is an enzyme that belongs to the ligase family. It can catalyze the connection between two RNA molecules, and a phosphodiester bond is formed during the connection process.
T4 RNA Ligase has a wide range of applications in molecular biology research. It is often used to construct RNA molecules, such as joining DNA to RNA molecules in synthetic molecular cloning, or joining two RNA molecules to construct longer RNA strands. In addition, T4 RNA Ligase can also be used to repair broken RNA strands or to help join modified RNA molecules in RNA modification studies.
The method of preparing T4 RNA Ligase usually involves extracting and purifying the enzyme from E. coli. Enzyme activity is typically measured by a substrate ligation reaction with phosphodiester bond formation. Commercial T4 RNA Ligase products are currently available for purchase for use in research laboratories.
Regarding safety information, T4 RNA Ligase is a natural enzyme extracted from bacteria and is generally low in toxicity. In the process of use, it is necessary to follow the laboratory's biosafety procedures. At the same time, pay attention to avoid enzyme contact with skin and eyes, avoid eating, inhalation. In case of accidental exposure, rinse immediately with plenty of water and consult a doctor.
Last Update:2024-04-09 21:11:58